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elisa hpasmcs  (Lonza)


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    Lonza elisa hpasmcs
    Overexpression of active YAP and TAZ represses COX-2. Human PASMCs (Lonza) were stably transfected with FLAG-tagged, nuclear-localizing YAP (YAP5SA) or TAZ (TAZ4SA), similar constructs lacking TEAD-binding capability (YAP5SA S94A, TAZ4SA S51A), or control vector (pLVX-Puro). RNA was isolated and assessed for YAP (A), TAZ (B), and COX-2 (C) expression via qPCR. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 2–4 independent experiments. D–H: protein was isolated and Western blot was performed for TAZ, YAP, ANKRD1, FLAG, and COX-2. Quantification represents 3 independent experiments; representative blots are shown. *P < 0.05 for YAP5SA compared with pLVX-Puro and TAZ4SA. **P < 0.05 for TAZ4SA compared with pLVX-Puro and YAP5SA. #P < 0.05 for TAZ4SA compared with pLVX-Puro. ¥P < 0.01 for pLVX-Puro compared with TAZ4SA and YAP5SA. I: levels of 6-keto-PGF1α were measured by <t>ELISA.</t> Statistical significance was determined by one-way ANOVA. *P = 0.02, **P = 0.009 compared with pLVX-Puro. #P = NS. n = 3 experiments. J: TAZ4SA-expressing cells and control (pLVX-Puro)-expressing cells were seeded onto discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kDa. 6-keto-PGF1α concentrations were measured by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 2 independent experiments.
    Elisa Hpasmcs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+hpasmcs/pmc05625262-120-0-2?v=Lonza
    Average 90 stars, based on 1 article reviews
    elisa hpasmcs - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Arterial stiffness induces remodeling phenotypes in pulmonary artery smooth muscle cells via YAP/TAZ-mediated repression of cyclooxygenase-2"

    Article Title: Arterial stiffness induces remodeling phenotypes in pulmonary artery smooth muscle cells via YAP/TAZ-mediated repression of cyclooxygenase-2

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    doi: 10.1152/ajplung.00173.2017

    Overexpression of active YAP and TAZ represses COX-2. Human PASMCs (Lonza) were stably transfected with FLAG-tagged, nuclear-localizing YAP (YAP5SA) or TAZ (TAZ4SA), similar constructs lacking TEAD-binding capability (YAP5SA S94A, TAZ4SA S51A), or control vector (pLVX-Puro). RNA was isolated and assessed for YAP (A), TAZ (B), and COX-2 (C) expression via qPCR. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 2–4 independent experiments. D–H: protein was isolated and Western blot was performed for TAZ, YAP, ANKRD1, FLAG, and COX-2. Quantification represents 3 independent experiments; representative blots are shown. *P < 0.05 for YAP5SA compared with pLVX-Puro and TAZ4SA. **P < 0.05 for TAZ4SA compared with pLVX-Puro and YAP5SA. #P < 0.05 for TAZ4SA compared with pLVX-Puro. ¥P < 0.01 for pLVX-Puro compared with TAZ4SA and YAP5SA. I: levels of 6-keto-PGF1α were measured by ELISA. Statistical significance was determined by one-way ANOVA. *P = 0.02, **P = 0.009 compared with pLVX-Puro. #P = NS. n = 3 experiments. J: TAZ4SA-expressing cells and control (pLVX-Puro)-expressing cells were seeded onto discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kDa. 6-keto-PGF1α concentrations were measured by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 2 independent experiments.
    Figure Legend Snippet: Overexpression of active YAP and TAZ represses COX-2. Human PASMCs (Lonza) were stably transfected with FLAG-tagged, nuclear-localizing YAP (YAP5SA) or TAZ (TAZ4SA), similar constructs lacking TEAD-binding capability (YAP5SA S94A, TAZ4SA S51A), or control vector (pLVX-Puro). RNA was isolated and assessed for YAP (A), TAZ (B), and COX-2 (C) expression via qPCR. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 2–4 independent experiments. D–H: protein was isolated and Western blot was performed for TAZ, YAP, ANKRD1, FLAG, and COX-2. Quantification represents 3 independent experiments; representative blots are shown. *P < 0.05 for YAP5SA compared with pLVX-Puro and TAZ4SA. **P < 0.05 for TAZ4SA compared with pLVX-Puro and YAP5SA. #P < 0.05 for TAZ4SA compared with pLVX-Puro. ¥P < 0.01 for pLVX-Puro compared with TAZ4SA and YAP5SA. I: levels of 6-keto-PGF1α were measured by ELISA. Statistical significance was determined by one-way ANOVA. *P = 0.02, **P = 0.009 compared with pLVX-Puro. #P = NS. n = 3 experiments. J: TAZ4SA-expressing cells and control (pLVX-Puro)-expressing cells were seeded onto discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kDa. 6-keto-PGF1α concentrations were measured by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 2 independent experiments.

    Techniques Used: Over Expression, Stable Transfection, Transfection, Construct, Binding Assay, Control, Plasmid Preparation, Isolation, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Shear

    YAP/TAZ knockdown enhances COX-2 expression and activity. Human PASMCs (Lonza) were grown on discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kPa with or without siControl or siYAP/TAZ transfection. A–E: RNA was isolated 48 h after treatment, and qPCR was performed to assess COX-2 (A and B), COX-1 (C), PTGER2 (D), and PTGIR (E) expression. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 6 independent experiments. No significant differences in COX-1, PTGER2, or PTGIR expression were observed. F and G: protein was isolated and Western blotting performed using anti-COX-2 (Santa Cruz) and anti-GAPDH (Santa Cruz) antibodies. n = 3 independent experiments, representative blots are shown. H: levels of 6-keto-PGF1α were measured in media by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 3 independent experiments.
    Figure Legend Snippet: YAP/TAZ knockdown enhances COX-2 expression and activity. Human PASMCs (Lonza) were grown on discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kPa with or without siControl or siYAP/TAZ transfection. A–E: RNA was isolated 48 h after treatment, and qPCR was performed to assess COX-2 (A and B), COX-1 (C), PTGER2 (D), and PTGIR (E) expression. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 6 independent experiments. No significant differences in COX-1, PTGER2, or PTGIR expression were observed. F and G: protein was isolated and Western blotting performed using anti-COX-2 (Santa Cruz) and anti-GAPDH (Santa Cruz) antibodies. n = 3 independent experiments, representative blots are shown. H: levels of 6-keto-PGF1α were measured in media by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 3 independent experiments.

    Techniques Used: Knockdown, Expressing, Activity Assay, Shear, Transfection, Isolation, Western Blot, Enzyme-linked Immunosorbent Assay



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    elisa hpasmcs  (Lonza)
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    Lonza elisa hpasmcs
    Overexpression of active YAP and TAZ represses COX-2. Human PASMCs (Lonza) were stably transfected with FLAG-tagged, nuclear-localizing YAP (YAP5SA) or TAZ (TAZ4SA), similar constructs lacking TEAD-binding capability (YAP5SA S94A, TAZ4SA S51A), or control vector (pLVX-Puro). RNA was isolated and assessed for YAP (A), TAZ (B), and COX-2 (C) expression via qPCR. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 2–4 independent experiments. D–H: protein was isolated and Western blot was performed for TAZ, YAP, ANKRD1, FLAG, and COX-2. Quantification represents 3 independent experiments; representative blots are shown. *P < 0.05 for YAP5SA compared with pLVX-Puro and TAZ4SA. **P < 0.05 for TAZ4SA compared with pLVX-Puro and YAP5SA. #P < 0.05 for TAZ4SA compared with pLVX-Puro. ¥P < 0.01 for pLVX-Puro compared with TAZ4SA and YAP5SA. I: levels of 6-keto-PGF1α were measured by <t>ELISA.</t> Statistical significance was determined by one-way ANOVA. *P = 0.02, **P = 0.009 compared with pLVX-Puro. #P = NS. n = 3 experiments. J: TAZ4SA-expressing cells and control (pLVX-Puro)-expressing cells were seeded onto discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kDa. 6-keto-PGF1α concentrations were measured by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 2 independent experiments.
    Elisa Hpasmcs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+hpasmcs/pmc05625262-120-0-2?v=Lonza
    Average 90 stars, based on 1 article reviews
    elisa hpasmcs - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    Overexpression of active YAP and TAZ represses COX-2. Human PASMCs (Lonza) were stably transfected with FLAG-tagged, nuclear-localizing YAP (YAP5SA) or TAZ (TAZ4SA), similar constructs lacking TEAD-binding capability (YAP5SA S94A, TAZ4SA S51A), or control vector (pLVX-Puro). RNA was isolated and assessed for YAP (A), TAZ (B), and COX-2 (C) expression via qPCR. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 2–4 independent experiments. D–H: protein was isolated and Western blot was performed for TAZ, YAP, ANKRD1, FLAG, and COX-2. Quantification represents 3 independent experiments; representative blots are shown. *P < 0.05 for YAP5SA compared with pLVX-Puro and TAZ4SA. **P < 0.05 for TAZ4SA compared with pLVX-Puro and YAP5SA. #P < 0.05 for TAZ4SA compared with pLVX-Puro. ¥P < 0.01 for pLVX-Puro compared with TAZ4SA and YAP5SA. I: levels of 6-keto-PGF1α were measured by ELISA. Statistical significance was determined by one-way ANOVA. *P = 0.02, **P = 0.009 compared with pLVX-Puro. #P = NS. n = 3 experiments. J: TAZ4SA-expressing cells and control (pLVX-Puro)-expressing cells were seeded onto discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kDa. 6-keto-PGF1α concentrations were measured by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 2 independent experiments.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Arterial stiffness induces remodeling phenotypes in pulmonary artery smooth muscle cells via YAP/TAZ-mediated repression of cyclooxygenase-2

    doi: 10.1152/ajplung.00173.2017

    Figure Lengend Snippet: Overexpression of active YAP and TAZ represses COX-2. Human PASMCs (Lonza) were stably transfected with FLAG-tagged, nuclear-localizing YAP (YAP5SA) or TAZ (TAZ4SA), similar constructs lacking TEAD-binding capability (YAP5SA S94A, TAZ4SA S51A), or control vector (pLVX-Puro). RNA was isolated and assessed for YAP (A), TAZ (B), and COX-2 (C) expression via qPCR. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 2–4 independent experiments. D–H: protein was isolated and Western blot was performed for TAZ, YAP, ANKRD1, FLAG, and COX-2. Quantification represents 3 independent experiments; representative blots are shown. *P < 0.05 for YAP5SA compared with pLVX-Puro and TAZ4SA. **P < 0.05 for TAZ4SA compared with pLVX-Puro and YAP5SA. #P < 0.05 for TAZ4SA compared with pLVX-Puro. ¥P < 0.01 for pLVX-Puro compared with TAZ4SA and YAP5SA. I: levels of 6-keto-PGF1α were measured by ELISA. Statistical significance was determined by one-way ANOVA. *P = 0.02, **P = 0.009 compared with pLVX-Puro. #P = NS. n = 3 experiments. J: TAZ4SA-expressing cells and control (pLVX-Puro)-expressing cells were seeded onto discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kDa. 6-keto-PGF1α concentrations were measured by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 2 independent experiments.

    Article Snippet: ELISA hPASMCs (Lonza) or stable YAP or TAZ overexpressing lines derived from hPASMCs (above) were seeded at a density of 100 cells/mm 2 in 24-well plates containing discrete stiffness polyacrylamide gels with stiffness of 0.4, 6.4, or 25.6 kPa or on TCP and cultured at 37°C and 5% CO 2 .

    Techniques: Over Expression, Stable Transfection, Transfection, Construct, Binding Assay, Control, Plasmid Preparation, Isolation, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Shear

    YAP/TAZ knockdown enhances COX-2 expression and activity. Human PASMCs (Lonza) were grown on discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kPa with or without siControl or siYAP/TAZ transfection. A–E: RNA was isolated 48 h after treatment, and qPCR was performed to assess COX-2 (A and B), COX-1 (C), PTGER2 (D), and PTGIR (E) expression. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 6 independent experiments. No significant differences in COX-1, PTGER2, or PTGIR expression were observed. F and G: protein was isolated and Western blotting performed using anti-COX-2 (Santa Cruz) and anti-GAPDH (Santa Cruz) antibodies. n = 3 independent experiments, representative blots are shown. H: levels of 6-keto-PGF1α were measured in media by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 3 independent experiments.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Arterial stiffness induces remodeling phenotypes in pulmonary artery smooth muscle cells via YAP/TAZ-mediated repression of cyclooxygenase-2

    doi: 10.1152/ajplung.00173.2017

    Figure Lengend Snippet: YAP/TAZ knockdown enhances COX-2 expression and activity. Human PASMCs (Lonza) were grown on discrete stiffness polyacrylamide gels with shear moduli of 0.4, 6.4, and 25.6 kPa with or without siControl or siYAP/TAZ transfection. A–E: RNA was isolated 48 h after treatment, and qPCR was performed to assess COX-2 (A and B), COX-1 (C), PTGER2 (D), and PTGIR (E) expression. Kruskal-Wallis ANOVA testing was performed on ΔCT values, and Dunn’s posttest was performed. Relative expression is displayed ± SD; n = 6 independent experiments. No significant differences in COX-1, PTGER2, or PTGIR expression were observed. F and G: protein was isolated and Western blotting performed using anti-COX-2 (Santa Cruz) and anti-GAPDH (Santa Cruz) antibodies. n = 3 independent experiments, representative blots are shown. H: levels of 6-keto-PGF1α were measured in media by ELISA and normalized to cell number. Statistical significance was determined by two-way ANOVA; n = 3 independent experiments.

    Article Snippet: ELISA hPASMCs (Lonza) or stable YAP or TAZ overexpressing lines derived from hPASMCs (above) were seeded at a density of 100 cells/mm 2 in 24-well plates containing discrete stiffness polyacrylamide gels with stiffness of 0.4, 6.4, or 25.6 kPa or on TCP and cultured at 37°C and 5% CO 2 .

    Techniques: Knockdown, Expressing, Activity Assay, Shear, Transfection, Isolation, Western Blot, Enzyme-linked Immunosorbent Assay